Anticorps Polyclonal de lapin anti-CCBL1/KAT1

CCBL1/KAT1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 12156-1-AP

Synonymes

CCBL1, CCBL1/KAT1, Glutamine transaminase K, GTK, KAT1, KATI, KYAT1, Kynurenine aminotransferase I

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Galerie de données de validation

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  WB analysis of mouse kidney using 12156-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12156-1-AP (CCBL1/KAT1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12156-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12156-1-AP (CCBL1/KAT1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12156-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12156-1-AP (CCBL1/KAT1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 12156-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12156-1-AP (CCBL1/KAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HeLa, cellules L02
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 1 publications below

Informations sur le produit

12156-1-AP cible CCBL1/KAT1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCBL1/KAT1 Protéine recombinante Ag2828
• Nom complet: cysteine conjugate-beta lyase, cytoplasmic
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC013069
• Symbole du gène: CCBL1
• Identification du gène (NCBI): 883
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCBL1 encodes kynurenine aminotransferase 1 (KAT1), a key enzyme involved in kynurenine pathway. CCBL1 catalyzes the production of kynurenic acid, a powerful endogenous excitatory amino acid receptor antagonist that is widely regarded as the potent neuroprotective agent.

Publications

Species	Application	Title
mouse	WB	Cell; Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience to stress-induced depression.; Authors - Leandro Z Agudelo
human	WB,IHC	J Intern Med; Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans.; Authors - R Baumgartner
mouse	WB	Brain Behav Immun; Kynurenine pathway is altered in BDNF Val66Met knock-in mice: Effect of physical exercise.; Authors - Alessandro Ieraci
		Am J Physiol Cell Physiol; Endurance exercise increases skeletal muscle kynurenine aminotransferases and plasma kynurenic acid in humans.; Authors - Maja Schlittler
mouse	WB	Sci Rep; Caffeine protects against stress-induced murine depression through activation of PPARγC1α-mediated restoration of the kynurenine pathway in the skeletal muscle.; Authors - Chongye Fang
human	WB	Exp Gerontol; Kynurenine aminotransferase isoforms display fiber-type specific expression in young and old human skeletal muscle.; Authors - V L Wyckelsma