Anticorps Polyclonal de lapin anti-CCAR1

CCAR1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 25232-1-AP

Synonymes

RP11 437A18.1, DIS, Death inducer with SAP domain, CARP-1, CARP1

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Galerie de données de validation

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  WB analysis using 25232-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25232-1-AP (CCAR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 25232-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (25232-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of A431 using 25232-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (25232-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer du sein humain, tissu de côlon de rat, tissu de côlon de souris, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25232-1-AP cible CCAR1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCAR1 Protéine recombinante Ag18970
• Nom complet: cell division cycle and apoptosis regulator 1
• Masse moléculaire calculée: 1150 aa, 133 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC132725
• Symbole du gène: CCAR1
• Identification du gène (NCBI): 55749
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cell cycle and apoptosis regulator 1 (CCAR1) was originally identified from a functional screening and implicated in retinoid-induced cell cycle arrest and apoptosis. It has been shown that, in breast cancer cells, CCAR1 regulates apoptosis induced by various agents, including chemotherapeutics adriamycin, etoposide, and EGFR inhibitor. It is also involved in mediating anchorage-independent growth of colon carcinoma cells. In addition, CCAR1 plays the role as a co-activator of nuclear receptors to recruit mediator complex. It was found to associate with components of the Mediator and p160 coactivator complexes and is recruited to endogenous NR target genes. CCAR1 is also a binding partner of b-catenin and assists b-catenin in transcriptional activation of Wnt target genes.