Anticorps Polyclonal de lapin anti-CC2D1B

• Phare
• Validé par KD/KO

CC2D1B Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA, IF

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 20774-1-AP

Synonymes

CC2D1B, Freud-2, KIAA1836, LGD1

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Galerie de données de validation

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  WB analysis of mouse brain using 20774-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20774-1-AP (CC2D1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20774-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20774-1-AP (CC2D1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20774-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20774-1-AP (CC2D1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 20774-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20774-1-AP (CC2D1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20774-1-AP

  IP result of anti-CC2D1B (IP:20774-1-AP, 4ug; Detection:20774-1-AP 1:500) with mouse brain tissue lysate 2560ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules SH-SY5Y, tissu placentaire humain, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

20774-1-AP cible CC2D1B dans les applications de WB, IP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CC2D1B Protéine recombinante Ag14767
• Nom complet: coiled-coil and C2 domain containing 1B
• Masse moléculaire calculée: 858 aa, 94 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC007912
• Symbole du gène: CC2D1B
• Identification du gène (NCBI): 200014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CC2D1B/Freud2 and CC2D1A/Freud1 are two homologs belonging to the evolutionary conserved Lgd protein family that is involved in endosomal trafficking. CC2D1B has been identified as the novel repressor of postsynaptic 5-HT1A expression. The predicted molecular weight of CC2D1B is 89 kDa, but the observed molecular weight is around 120 kDa, which may be caused by post-translational modification. This antibody is specific to CC2D1B, which has been confirmed by siRNA test. (19423080 , 2672064)

Publications

Species	Application	Title
mouse	WB,IF	PLoS Genet; The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling.; Authors - Nadja Drusenheimer ; KO Validated
mouse	WB	Front Mol Neurosci; Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System.; Authors - Jenica Acheta ; KO Validated
rat	WB,IF	Front Mol Neurosci; YAP and TAZ Regulate Cc2d1b and Purβ in Schwann Cells.; Authors - Belin Sophie ; KD Validated
human	IF	Biol Pharm Bull; Icariin regulates EMT and stem cell-like character in breast cancer through modulating lncRNA NEAT1/TGFβ/SMAD2 signaling pathway; Authors - Bo Song
human	WB	Nat Commun; Replication competent HIV-guided CRISPR screen identifies antiviral factors including targets of the accessory protein Nef; Authors - Caterina Prelli Bozzo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Abigail (Verified Customer) (09-16-2019)

It worked well for us

• Applications: Western Blot, Immunoprecipitation,
• Cell Tissue Type: Murine Hippocampus and cortex