Anticorps Polyclonal de lapin anti-CBX2

• Phare
• Validé par KD/KO

CBX2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 15579-1-AP

Synonymes

CBX2, CDCA6, Chromobox protein homolog 2, M33

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Galerie de données de validation

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  WB analysis using 15579-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15579-1-AP (CBX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15579-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15579-1-AP (CBX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15579-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15579-1-AP (CBX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules MCF-7, tissu cardiaque de rat, tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

15579-1-AP cible CBX2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CBX2 Protéine recombinante Ag7979
• Nom complet: chromobox homolog 2 (Pc class homolog, Drosophila)
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC004252
• Symbole du gène: CBX2
• Identification du gène (NCBI): 84733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The chromobox homologue protein 2 (CBX2) is a critical component of the PRC1 complex involved in antiviral innate immunity, neuronal, and gonadal differentiation, axial patterning during embryonic development, cell proliferation, and senescence (PMID: 32870972). CBX2 has 2 isoforms with the molecular mass of 23 and 56 kDa. Sometimes higher molecular weight around 65-70 kDa can also be observed, which may be a modified variant of CBX2.

Publications

Species	Application	Title
human	WB	Cell; Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.; Authors - Xun Huang ; KD Validated
human	WB,IHC	Am J Transl Res; Overexpression of CBX2 in breast cancer promotes tumor progression through the PI3K/AKT signaling pathway.; Authors - Shipeng Zheng ; KD Validated
human	WB,IHC,IF	Mol Carcinog; Loss of CBX2 causes genomic instability and Wnt activation in high grade serous ovarian carcinoma cells; Authors - Yujia Ma ; KD Validated
mouse	WB	Cell Rep; SENP3 and USP7 regulate Polycomb-rixosome interactions and silencing functions; Authors - Haining Zhou
human	WB	Oncol Lett; Comprehensive analysis reveals the value of the expression of chromobox family members for bladder urothelial carcinoma prognosis; Authors - Xuan Meng
human	WB	Heliyon; Derepressing of STAT3 and USP7 contributes to resistance of DLBCL to EZH2 inhibition; Authors - Chenyun He