Anticorps Polyclonal de lapin anti-CBLB

• Phare
• Validé par KD/KO

CBLB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 12781-1-AP

Synonymes

RING-type E3 ubiquitin transferase CBL-B, RING finger protein 56, Nbla00127, EC:2.3.2.27, E3 ubiquitin-protein ligase CBL-B

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Galerie de données de validation

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  WB analysis of HEK-293 using 12781-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12781-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12781-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12781-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12781-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12781-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12781-1-AP

  IP result of anti-CBLB (IP:12781-1-AP, 4ug; Detection:12781-1-AP 1:600) with HEK-293 cells lysate 1800 ug.

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  IHC staining of mouse colon using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12781-1-AP (CBLB antibody) at dilution of 1:50 (under 10x lens)..

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  IHC staining of mouse colon using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12781-1-AP (CBLB antibody) at dilution of 1:50 (under 40x lens)..

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  IHC staining of human lung using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A431 using 12781-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBLB antibody (12781-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de côlon de souris, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 3 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12781-1-AP cible CBLB dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CBLB Protéine recombinante Ag3491
• Nom complet: Cas-Br-M (murine) ecotropic retroviral transforming sequence b
• Masse moléculaire calculée: 982 aa, 109 kDa
• Poids moléculaire observé: 110-130 kDa
• Numéro d’acquisition GenBank: BC032851
• Symbole du gène: CBLB
• Identification du gène (NCBI): 868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CBLB(E3 ubiquitin-protein ligase CBL-B) is also named RNF56 and plays a necessary role in preventing the inappropriate activation of NF-B in response to lipopolysaccharide and polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and various types of cell stimulation. It also has a crucial role in preventing chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing 109, 90, and 86 kDa, and observed molecular weight at 110-130 kDa.

Publications

Species	Application	Title
mouse	WB	J Exp Med; Cbl-b deficiency prevents functional but not phenotypic T cell anergy.; Authors - Trang T T Nguyen
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
rat	IHC	Sci Signal; Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.; Authors - Zi-Yang Zhang
human	FC	J Immunol; A Multiple Sclerosis-Associated Variant of CBLB Links Genetic Risk with Type I IFN Function.; Authors - Klarissa Hanja Stürner
mouse	WB,IF	J Cell Mol Med; NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.; Authors - Ze-Qing Pu
mouse	WB	Biomolecules; miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles.; Authors - Xin Yang