Anticorps Polyclonal de lapin anti-Caspase 2/p32/p18

Caspase 2/p32/p18 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IP, RIP, ELISA, IF

Conjugaison

Non conjugué

Publications

8

N° de cat : 10436-1-AP

Synonymes

CASP 2, CASP2, Caspase 2, Caspase 2/p18, Caspase 2/p32/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1

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Galerie de données de validation

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  WB analysis of Jurkat using 10436-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (Caspase 2/p18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10436-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10436-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10436-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10436-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10436-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10436-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10436-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10436-1-AP

  IP result of anti-Caspase 2/p32/p18 (IP:10436-1-AP, 4ug; Detection:10436-1-AP 1:2000) with HeLa cells lysate 3000 ug.

• 

  IHC staining of human kidney using 10436-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10436-1-AP (Caspase 2/p18 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10436-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10436-1-AP (Caspase 2/p18 antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat traitées à la staurosporine, cellules HEK-293, cellules HeLa, cellules HL-60, cellules Jurkat, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

10436-1-AP cible Caspase 2/p32/p18 dans les applications de WB, IHC, IP, RIP, ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Caspase 2/p32/p18 Protéine recombinante Ag0694
• Nom complet: caspase 2, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 452 aa, 51 kDa
• Poids moléculaire observé: 48-51 kDa, 32 kDa, 18kDa
• Numéro d’acquisition GenBank: BC002427
• Symbole du gène: Caspase 2
• Identification du gène (NCBI): 835
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).

Publications

Species	Application	Title
human	WB, IF	Front Microbiol; Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.; Authors - Bei Wang
		Front Pharmacol; PMID: 32210806; Authors - Wen Su
rabbit	RIP	Life Sci; Timolol induces necroptosis, apoptosis and senescence concentration-dependently in rabbit Limbal stem cells in vitro.; Authors - Guo-Jian Jiang
human	ELISA	Toxicol In Vitro; PMID: 32320760; Authors - Ting Yang
mouse	WB	Toxicon; Involvement of caspase in patulin-induced hepatotoxicity in vitro and in vivo.; Authors - Baigang Zhang
		Chem Biol Interact; Different concentrations of betaxolol switch cell fate between necroptosis, apoptosis, and senescence in human corneal stromal cells; Authors - Jing-Yu Sun