Anticorps Polyclonal de lapin anti-Caspase 12

• Phare
• Validé par KD/KO

Caspase 12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

91

N° de cat : 55238-1-AP

Synonymes

CASP 12, CASP12, CASP12P1, Caspase 12, caspase 12 (gene/pseudogene), Caspase12, Inactive caspase 12

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Galerie de données de validation

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  WB analysis of HEK-293 using 55238-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells using 55238-1-AP

  WB result of 55238-1-AP (CASP12 antibody) with various lysates.

• 

  WB analysis of L02 using 55238-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55238-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55238-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 55238-1-AP

  IP Result of anti-Caspase 12 (IP:55238-1-AP, 4ug; Detection:55238-1-AP 1:1000) with HEK-293 cells lysate 2000ug.

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  IHC staining of human prostate hyperplasia using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55238-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 55238-1-AP (Caspase 12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, multi-cellules
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu d'hyperplasie de la prostate humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 88 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

55238-1-AP cible Caspase 12 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: caspase 12 (gene/pseudogene)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 36-42 kDa, 50 kDa
• Numéro d’acquisition GenBank: NR_000035
• Symbole du gène: CASP12
• Identification du gène (NCBI): 120329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases.

Publications

Species	Application	Title
human	WB	Autophagy; SARS-CoV-2 ORF3a induces RETREG1/FAM134B-dependent reticulophagy and triggers sequential ER stress and inflammatory responses during SARS-CoV-2 infection.; Authors - Xiaolin Zhang
human	WB	ACS Appl Mater Interfaces; Endoplasmic Reticulum-Targeted Aggregation-Induced Emission Luminogen for Synergetic Tumor Ablation with Glibenclamide; Authors - Yifan Wu
	WB	J Hazard Mater; Perinatal low-dose PBDE-47 exposure hampered thyroglobulin turnover and induced thyroid cell apoptosis by triggering ER stress and lysosomal destabilization contributing to thyroid toxicity in adult female rats.; Authors - Pei Li
human	WB,IF	Theranostics; Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo.; Authors - Zhiwei Liao
human	WB	Biomed Pharmacother; A novel synthetic curcumin derivative MHMM-41 induces ROS-mediated apoptosis and migration blocking of human lung cancer cells A549.; Authors - Guang-Zhou Zhou
human	WB	Oxid Med Cell Longev; Betulinic Acid Suppresses Breast Cancer Metastasis by Targeting GRP78-Mediated Glycolysis and ER Stress Apoptotic Pathway.; Authors - Yifeng Zheng

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