Anticorps Polyclonal de lapin anti-MLN51/CASC3

MLN51/CASC3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 18047-1-AP

Synonymes

BTZ, CASC3, MLN 51, MLN51, Protein barentsz, Protein CASC3

---

Galerie de données de validation

• 

  WB analysis using 18047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18047-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 18047-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18047-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18047-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18047-1-AP

  Immunofluorescent analysis of HepG2 cells, using CASC3 antibody 18047-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: cellules A2780, cellules MCF-7, cellules SKOV-3
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

18047-1-AP cible MLN51/CASC3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLN51/CASC3 Protéine recombinante Ag12694
• Nom complet: cancer susceptibility candidate 3
• Masse moléculaire calculée: 703 aa, 76 kDa
• Poids moléculaire observé: 115-120 kDa
• Numéro d’acquisition GenBank: BC050526
• Symbole du gène: CASC3
• Identification du gène (NCBI): 22794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CASC3(cancer susceptibility candidate 3) is also named as MLN51 and belongs to the CASC3 family. It is induced by CSF2, and is important in the proliferation of RA fibroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis (RA)(PMID:17101062). It is a nucleocytoplasmic protein containing, within its aminoterminal half, a coiled-coil domain followed by two nuclear localization signals (NLSs) responsible for nuclear localization and, in its carboxyl-terminal half, a nuclear export signal(NES) that mediates cytoplasmic retention. It possesses many potential phosphorylation sites and is found to be phosphorylated in both normal and cancer-derived cells.(PMID:17652158).

Publications

Species	Application	Title
human	WB	Mol Cell; Identification of a microRNA that activates gene expression by repressing nonsense-mediated RNA decay.; Authors - Ivone G Bruno
human	WB,IF	Mol Biol Cell; Perispeckles are major assembly sites for the exon junction core complex.; Authors - Daguenet Elisabeth E