Anticorps Polyclonal de lapin anti-CARD9

• Phare
• Validé par KD/KO

CARD9 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 10669-1-AP

Synonymes

CARD9, hCARD9

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Galerie de données de validation

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  WB analysis of HL-60 using 10669-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10669-1-AP (CARD9 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10669-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10669-1-AP (CARD9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IP: See 1 publications below

Informations sur le produit

10669-1-AP cible CARD9 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CARD9 Protéine recombinante Ag1057
• Nom complet: caspase recruitment domain family, member 9
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 50-59 kDa
• Numéro d’acquisition GenBank: BC008877
• Symbole du gène: CARD9
• Identification du gène (NCBI): 64170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in spleen and regulate activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.

Publications

Species	Application	Title
human	WB	Blood; Invasive fungal infection and impaired neutrophil killing in human CARD9 deficiency.; Authors - Drewniak Agata A
human	WB	PLoS Pathog; CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System.; Authors - Rebecca A Drummond
human	WB	J Clin Immunol; Inherited CARD9 Deficiency in a Child with Invasive Disease Due to Exophiala dermatitidis and Two Older but Asymptomatic Siblings.; Authors - Yusuke Imanaka
mouse	WB	Transl Stroke Res; Inhibition of Dectin-1 Ameliorates Neuroinflammation by Regulating Microglia/Macrophage Phenotype After Intracerebral Hemorrhage in Mice.; Authors - Xiongjie Fu
human	WB	Plast Reconstr Surg; Chronic Candida albicans Meningitis in a 4-Year-Old Girl with a Homozygous Mutation in the CARD9 Gene (Q295X).; Authors - Martin Herbst
mouse	WB	Front Immunol; TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage.; Authors - Xinyan Wu