Anticorps Polyclonal de lapin anti-CARD6

CARD6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 18029-1-AP

Synonymes

CARD6, CINCIN1

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Galerie de données de validation

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  WB analysis of HepG2 using 18029-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18029-1-AP (CARD6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2
• Résultats positifs en IHC: tissu testiculaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

18029-1-AP cible CARD6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CARD6 Protéine recombinante Ag12595
• Nom complet: caspase recruitment domain family, member 6
• Masse moléculaire calculée: 1037 aa, 116 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC093825
• Symbole du gène: CARD6
• Identification du gène (NCBI): 84674
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The caspase recruitment domain (CARD) is a homotypic protein-protein interaction module that links components of signal transduction pathways implicated in the regulation of apoptosis or adaptive or innate immunity. Although much progress has been made in assigning precise roles to most CARD-containing proteins, the functions of the 1,037-amino-acid (aa) human and 1,175-aa mouse CARD6 proteins are still unknown. CARD6 has a unique structure in that it contains the CARD at the N terminus, a glutamic acid-rich region following the CARD, and a proline-rich region at the C terminus. CARD6 also harbors a 350-aa region with similarity to upregulated gene 4 (URG4), a protein that is induced in response to hepatitis Bx antigen overexpression and exerts a positive effect on proliferation. Both CARD6 and URG4 share structural features with members of the multifaceted, IFN-inducible GTPase (IFNiGTPase) superfamily, which contains some of the proteins most abundantly induced during cell-autonomous immune responses. The calcualted molecular weight of CARD is 116 kDa, but modified CARD6 is about 130 kDa. (PMID: 18160713)

Publications

Species	Application	Title
rat	WB	J Cell Mol Med; Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.; Authors - Yong Zhang