Anticorps Polyclonal de lapin anti-CARD11

• Phare
• Validé par KD/KO

CARD11 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 21741-1-AP

Synonymes

BIMP3, CARD11, Carma 1, CARMA1

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Galerie de données de validation

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  WB analysis using 21741-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21741-1-AP (CARD11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 21741-1-AP

  WB result of CARD11 antibody (21741-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARD11 transfected Raji cells.

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  WB analysis of mouse thymus using 21741-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21741-1-AP (CARD11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 21741-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21741-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 21741-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of L02 using 21741-1-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using CARD11 antibody (21741-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Raji, tissu de thymus de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules L02,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

21741-1-AP cible CARD11 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CARD11 Protéine recombinante Ag16468
• Nom complet: caspase recruitment domain family, member 11
• Masse moléculaire calculée: 1154 aa, 133 kDa
• Poids moléculaire observé: 133 kDa
• Numéro d’acquisition GenBank: BC111719
• Symbole du gène: CARD11
• Identification du gène (NCBI): 84433
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.

Publications

Species	Application	Title
human	WB,IF	Front Immunol; Identification and Characterization of a Germline Mutation in CARD11 From a Chinese Case of B Cell Expansion With NF-κB and T Cell Anergy.; Authors - Peiwei Zhao
human	WB,IF	Front Immunol; A novel CARD11 germline mutation in a Chinese patient of B cell expansion with NF-κB and T cell anergy (BENTA) and literature review; Authors - Peiwei Zhao
mouse	WB	Immunobiology; Transcription suppression of GABARAP mediated by lncRNA XIST-EZH2 interaction triggers caspase-11-dependent inflammatory injury in ulcerative colitis; Authors - Dan Gu