Anticorps Polyclonal de lapin anti-CAPZB

• Phare
• Validé par KD/KO

CAPZB Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 25043-1-AP

Synonymes

CAPB, CAPPB, CAPZ, CapZ beta, CAPZB

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Galerie de données de validation

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  WB analysis using 25043-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25043-1-AP (CAPZB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25043-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25043-1-AP (CAPZB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 25043-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25043-1-AP (CAPZB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 25043-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 25043-1-AP (CAPZB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules NIH/3T3, tissu cardiaque de rat, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

25043-1-AP cible CAPZB dans les applications de WB, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAPZB Protéine recombinante Ag19327
• Nom complet: capping protein (actin filament) muscle Z-line, beta
• Masse moléculaire calculée: 277 aa, 31 kDa
• Poids moléculaire observé: 30-31 kDa
• Numéro d’acquisition GenBank: BC109241
• Symbole du gène: CAPZB
• Identification du gène (NCBI): 832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Cell Rep; Nanoscale Architecture of the Cortical Actin Cytoskeleton in Embryonic Stem Cells.; Authors - Shumin Xia ; KD Validated
human	WB,IP	Elife; Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.; Authors - Dawei Wang ; KD Validated
mouse	WB	Development; Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice.; Authors - Xiao-Zhen Zhang
human,mouse	CoIP	Cell Rep; The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice.; Authors - Xiao-Zhen Zhang
human	WB	Cell Rep; Substrate rigidity dictates colorectal tumorigenic cell stemness and metastasis via CRAD-dependent mechanotransduction.; Authors - Yuhan Chang
human	WB	BMC Biol; The temporal association of CapZ with early endosomes regulates endosomal trafficking and viral entry into host cells; Authors - Huazhang Zhu