Anticorps Polyclonal de lapin anti-Calpain 9

Calpain 9 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 17556-1-AP

Synonymes

Calpain 9, CAPN9, GC36, nCL 4, NCL4, New calpain 4, Protein CG36

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Galerie de données de validation

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  WB analysis of human stomach using 17556-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 17556-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 17556-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17556-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 17556-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17556-1-AP

  Immunofluorescent analysis of HepG2 cells, using CAPN9 antibody 17556-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu d'estomac humain, cellules HL-60, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu d'estomac humain, tissu ovarien humain, tissu pulmonaire humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

17556-1-AP cible Calpain 9 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calpain 9 Protéine recombinante Ag11718
• Nom complet: calpain 9
• Masse moléculaire calculée: 627 aa, 72 kDa
• Poids moléculaire observé: 60-66 kDa
• Numéro d’acquisition GenBank: BC027993
• Symbole du gène: Calpain 9
• Identification du gène (NCBI): 10753
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calpain 9 is also named as NCL4 and belongs to the peptidase C2 family.It can be down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types.It is predominantly expressed in the gastrointestinal tract and restricted to the gastric surface mucus (pit) cells in the stomach and the function of it is implicated in suppressing tumorigenesis(PMID:20686710).

Publications

Species	Application	Title
human,mouse,rat	WB,IF	Front Pharmacol; Inhibition of calpain9 attenuates peritoneal dialysis-related peritoneal fibrosis; Authors - Fang Li
human	WB,IHC	Sci Rep; Decreased expression of Calpain-9 predicts unfavorable prognosis in patients with gastric cancer.; Authors - Peike Peng