Anticorps Polyclonal de lapin anti-Calpain 2

• Phare
• Validé par KD/KO

Calpain 2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

15

Avis (1)

N° de cat : 11472-1-AP

Synonymes

Calpain 2, Calpain 2 catalytic subunit, Calpain 2 large subunit, Calpain large polypeptide L2, Calpain M type, CANP 2, CANP2, CANPL2, CANPml, CAPN2, M calpain, mCANP, Millimolar calpain

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Galerie de données de validation

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  WB analysis using 11472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-231 using 11472-1-AP

  MDA-MB-231 (WT and CAPN2 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 11472-1-AP incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HeLa using 11472-1-AP

  WB result of Calpain 2 antibody (11472-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpain 2 transfected HeLa cells.

• 

  WB analysis using 11472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11472-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 11472-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MDA-MB-231 using 11472-1-AP

  MDA-MB-231 lysates prepared and IP of CAPN2 performed using 1.0 μg of 11472-1-AP coupled to protein A- Sepharose beads. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human kidney using 11472-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11472-1-AP (Calpain 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11472-1-AP (Calpain 2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11472-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11472-1-AP (Calpain 2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 11472-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11472-1-AP (Calpain 2 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 11472-1-AP

  Immunofluorescent analysis of HepG2 cells, using CAPN2 antibody 11472-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of MDA-MB-231 using 11472-1-AP

  MDA-MB-231 WT cells (yellow outline) and SYT1 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 11472-1-AP at 1:300, plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules C2C12, cellules HeLa, cellules K-562, cellules MDA-MB-231, cellules PC-3, cellules Y79, tissu cérébral de rat, tissu cérébral de souris, tissu placentaire humain
• Résultats positifs en IP: cellules MDA-MB-231,
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules MDA-MB-231

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

11472-1-AP cible Calpain 2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calpain 2 Protéine recombinante Ag2029
• Nom complet: calpain 2, (m/II) large subunit
• Masse moléculaire calculée: 700 aa, 80 kDa
• Poids moléculaire observé: 72-80 kDa
• Numéro d’acquisition GenBank: BC021303
• Symbole du gène: CAPN2
• Identification du gène (NCBI): 824
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.

Publications

Species	Application	Title
human	WB,IHC	Phytomedicine; Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells; Authors - Maolei Xu
mouse	WB	Pharmacol Res; Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.; Authors - Juan Wang
mouse	IF	Pharmacol Res; TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.; Authors - Wen Tian
mouse	WB	J Am Heart Assoc; Cardiac Myocyte-Specific Overexpression of FASTKD1 Prevents Ventricular Rupture After Myocardial Infarction; Authors - Kurt D Marshall
rat	WB	Front Pharmacol; Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca2+ Signaling Pathway.; Authors - Lei Liu
Rat	WB,IHC	Int Immunopharmacol; Inhibition of extracellular signal-regulated kinase/calpain-2 pathway reduces neuroinflammation and necroptosis after cerebral ischemia-reperfusion injury in a rat model of cardiac arrest.; Authors - Wen-Yan Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (03-12-2020)

Weak but clear band for western blot on mouse heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart