Anticorps Polyclonal de lapin anti-CAPG-Specific

• Phare
• Validé par KD/KO

CAPG-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 19535-1-AP

Synonymes

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

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Galerie de données de validation

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  WB analysis of mouse lung using 19535-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19535-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 19535-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 19535-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 19535-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach cancer using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules U-937, tissu rénal de souris
• Résultats positifs en IHC: tissu splénique humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

19535-1-AP cible CAPG-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: capping protein (actin filament), gelsolin-like
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: NM_001747
• Symbole du gène: CAPG
• Identification du gène (NCBI): 822
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

Publications

Species	Application	Title
human	WB	Tohoku J Exp Med; Overexpression of gelsolin-like actin-capping protein is associated with progression of lung adenocarcinoma.; Authors - Shao Fangchun F ; KD Validated