Anticorps Polyclonal de lapin anti-CAP2

• Phare
• Validé par KD/KO

CAP2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 15865-1-AP

Synonymes

Adenylyl cyclase associated protein 2, CAP 2, CAP2

---

Galerie de données de validation

• 

  WB analysis of human kidney using 15865-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15865-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 15865-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15865-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15865-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15865-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15865-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15865-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15865-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 15865-1-AP

  IP result of anti-CAP2 (IP:15865-1-AP, 4ug; Detection:15865-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 15865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 15865-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15865-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of U2OS using 15865-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 15865-1-AP (CAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U2OS using 15865-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CAP2 antibody (15865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HepG2, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu rénal de souris, tissu rénal humain, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

15865-1-AP cible CAP2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAP2 Protéine recombinante Ag8477
• Nom complet: CAP, adenylate cyclase-associated protein, 2 (yeast)
• Masse moléculaire calculée: 477 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC008481
• Symbole du gène: CAP2
• Identification du gène (NCBI): 10486
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CAPs (CAP1 and CAP2) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is predominantly expressed in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Overexpression of CAP2 has been reported in many cancers, including hepatocellular carcinoma (HCC), human breast cancer, and malignant melanoma.

Publications

Species	Application	Title
human	WB,IHC,IF,CoIP	J Clin Invest; CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages; Authors - Guohao Zhang ; KD Validated
human	WB	Int J Mol Sci; Analysis of mRNA and Protein Levels of CAP2, DLG1 and ADAM10 Genes in Post-Mortem Brain of Schizophrenia, Parkinson's and Alzheimer's Disease Patients.; Authors - Anna Di Maio
mouse,rat	WB,IF	Commun Biol; CAP2 is a regulator of actin pointed end dynamics and myofibrillogenesis in cardiac muscle.; Authors - Mert Colpan ; KO Validated
mouse	WB	Sci Rep; Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblasts.; Authors - Lara-Jane Kepser
		Oncol Rep; Expression status of cyclase‑associated protein 2 as a prognostic marker for human breast cancer.; Authors - Lihua Xu
mouse	WB	Sci Signal; The actin-binding protein CAP1 represses MRTF-SRF-dependent gene expression in mouse cerebral cortex; Authors - Sharof Khudayberdiev

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Anil K (Verified Customer) (06-10-2022)

Saw a single band in the mice brain lysates

• Applications: Western Blot
• Cell Tissue Type: brain