Anticorps Polyclonal de lapin anti-CAMKV

CAMKV Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 14788-1-AP

Synonymes

1G5, CAMKV, VACAMKL

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Galerie de données de validation

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  WB analysis of mouse brain using 14788-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14788-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14788-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14788-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14788-1-AP

  IP result of anti-CAMKV (IP:14788-1-AP, 4ug; Detection:14788-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IF Staining of mouse brain using 14788-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14788-1-AP (CAMKV antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules PC-3, tissu splénique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14788-1-AP cible CAMKV dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAMKV Protéine recombinante Ag6438
• Nom complet: CaM kinase-like vesicle-associated
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54-75 kDa
• Numéro d’acquisition GenBank: BC000497
• Symbole du gène: CAMKV
• Identification du gène (NCBI): 79012
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CAMKV(CaM kinase-like vesicle-associated protein) is also named as 1G5,VACAMKL and belongs to the CAMK Ser/Thr protein kinase family. The CAMKV protein, which accumulates predominantly during postnatal development, binds to calmodulin in the presence of calcium. It is detected only in the CNS, is absent from cerebellum, but is detected in most other brain regions, especially regions of the cortex. The protein is predominantly detected in association with the plasma membrane of somatic cell and in neurites, both axons and dendrites, and is frequently observed in active zones, usually associated with vesicle-like structures. Its sequence suggests that lG5 may be membrane associated(PMID:8283228).

Publications

Species	Application	Title
mouse	WB	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen
human,mouse	WB,IHC,IF	Ann Neurol; Paraneoplastic Calmodulin Kinase-Like Vesicle-Associated Protein (CAMKV) Autoimmune Encephalitis; Authors - Michael Gilligan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

María Inmaculada (Verified Customer) (07-15-2022)

I used the antibody for Western Blot and it worked very well. First, we used it at a 1:500 dilution first, but it was super concentrated and obtained unspecific bands. Then, I used it at a 1:2500 dilution and obtained pretty clean bands for the protein. The signal was very intense with my system, so I think I could have even diluted it a bit more.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: Mouse brain