Anticorps Polyclonal de lapin anti-CaMKIV

CaMKIV Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13263-1-AP

Synonymes

CaM kinase GR, CaMK GR, CAMK4, CaMKIV

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Galerie de données de validation

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  WB analysis of human cerebellum using 13263-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13263-1-AP (CaMKIV antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13263-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13263-1-AP (CaMKIV antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 13263-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13263-1-AP (CaMKIV antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13263-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13263-1-AP (CaMKIV antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13263-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13263-1-AP (CaMKIV antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 13263-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13263-1-AP (CaMKIV antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de cervelet humain, tissu de thymus de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

13263-1-AP cible CaMKIV dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CaMKIV Protéine recombinante Ag3922
• Nom complet: calcium/calmodulin-dependent protein kinase IV
• Masse moléculaire calculée: 473 aa, 52 kDa
• Poids moléculaire observé: 56-65 kDa
• Numéro d’acquisition GenBank: BC025687
• Symbole du gène: CAMK4
• Identification du gène (NCBI): 814
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CaMK4 (Calcium/calmodulin-dependent protein kinase IV) is a multifunctional serine/threonine kinase that regulates gene expression by activating transcription factors in a wide range of immune cells including T cells and antigen-presenting cells. The function of CaMK4 is suggested to be abnormal mainly in systemic lupus erythematosus (SLE), which is characterized by autoantibody production, immune complex formation, and immune dysregulation (PMID: 29252071). CaMKIV can translocate between the nucleus and cytoplasm (PMID: 21301225)

Publications

Species	Application	Title
human	WB	Oncotarget; PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.; Authors - Chao Li
human	WB	J Invest Dermatol; The Long Noncoding RNA UCA1 Negatively Regulates Melanogenesis in Melanocytes.; Authors - Shiyao Pei
mouse	WB	Toxicol Res (Camb); Neurotoxicity, behavioral changes and gene-expression profile of mice exposed to SnS2 nanoflowers.; Authors - Disi Bai
rat	WB,IF	Cell Death Discov; Congenital hypothyroidism impairs spine growth of dentate granule cells by downregulation of CaMKIV.; Authors - Qingying Tang
rat	WB	Fitoterapia; Investigation of the underlying mechanism of Buyang Huanwu decoction in ischemic stroke by integrating systems pharmacology-proteomics and in vivo experiments; Authors - Weiguo Liao