Anticorps Polyclonal de lapin anti-Calmodulin

Calmodulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 10541-1-AP

Synonymes

Calmodulin 3, Calmodulin 1/2/3, CALML2, CALM3, CAM3

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Galerie de données de validation

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  WB analysis using 10541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10541-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10541-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 10541-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10541-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10541-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Ramos using 10541-1-AP

  IP result of anti-Calmodulin (IP:10541-1-AP, 2ug; Detection:10541-1-AP 1:600) with Ramos cells lysate 1120 ug.

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  IHC staining of human breast cancer using 10541-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10541-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10541-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10541-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MCF-7 using 10541-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Calmodulin (10541-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 10541-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Calmodulin (10541-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, cellules HeLa, cellules HepG2, cellules MCF-7, cellules NCCIT, tissu cérébral de souris, tissu de muscle squelettique de rat
• Résultats positifs en IP: cellules Ramos,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 2 publications below
• IP: See 2 publications below

Informations sur le produit

10541-1-AP cible Calmodulin dans les applications de WB, IHC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calmodulin Protéine recombinante Ag0827
• Nom complet: calmodulin 3 (phosphorylase kinase, delta)
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC006182
• Symbole du gène: Calmodulin 3
• Identification du gène (NCBI): 808
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calmodulin (CaM) is a Ca(2+)-binding protein that transduces Ca2+-mediated signals by binding to and regulating the activity of hundreds of enzymes and non-enzymatic proteins. It is highly conserved across species and involved in many biological processes, including vesicle release, cell proliferation, and apoptosis. This antibody can recognize CALM1, CALM2 and CALM3.

Publications

Species	Application	Title
human	WB	Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
human		Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
human	IHC, WB	Cancer Cell Int; CALM1 promotes progression and dampens chemosensitivity to EGFR inhibitor in esophageal squamous cell carcinoma.; Authors - Tao Liu
	IP	Neurochem Int; Alpha-synuclein overexpression increases phospho-protein phosphatase 2A levels via formation of calmodulin/Src complex.; Authors - W Yang
human	WB	Biochem Biophys Res Commun; The NMDAR GluN1-1a C-terminus binds to CaM and regulates synaptic function.; Authors - Liang Zhou
human	WB	J Cardiovasc Transl Res; TRPC1 Deficiency Impairs the Endothelial Progenitor Cell Function via Inhibition of Calmodulin/eNOS Pathway.; Authors - Lai-Ling Du