Anticorps Polyclonal de lapin anti-CA9

• Phare
• Validé par KD/KO

CA9 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 11443-1-AP

Synonymes

CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250

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Galerie de données de validation

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  WB analysis of A549 using 11443-1-AP

  WB result of CA9 antibody (11443-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA9 transfected A549 cells.

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  WB analysis of mouse brain using 11443-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11443-1-AP (CA IX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 11443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11443-1-AP (CA9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11443-1-AP (CA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

11443-1-AP cible CA9 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CA9 Protéine recombinante Ag1963
• Nom complet: carbonic anhydrase IX
• Masse moléculaire calculée: 459 aa, 50 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC014950
• Symbole du gène: CA9
• Identification du gène (NCBI): 768
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (10-28-2022)

Western blot, 30 µg lysate per well. Glioblastoma stem-like cells were cultured as small speroids and treated with CoCl2 to stabilise HIF-1a, or as medium or XL speroids (no CoCl2 treatment). There were are a few bands visible on the blot, but CoCl2 induced a band at around 60 kDa, and XL speroids which have a hypoxic core also resulted in a heavy band at the same height. See also: https://www.nature.com/articles/s41389-022-00433-3#Sec18 Figure S5 and supplementary methods for WB details.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma stem-like cells

CA9 Antibody Western Blot validation (1:1000 dilution) in Glioblastoma stem-like cells (Cat no:11443-1-AP)