• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-CA3

CA3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 15197-1-AP

Synonymes

CA III, CA3, CAIII, Car3, Carbonate dehydratase III, Carbonic anhydrase 3, Carbonic anhydrase III



Applications testées

Résultats positifs en WBcellules HEK-293, cellules HeLa, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
Résultats positifs en IHCtissu de muscle squelettique de souris,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF-Ptissu de muscle squelettique de souris, tissu rénal humain

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochimie (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15197-1-AP cible CA3 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène CA3 Protéine recombinante Ag7344
Nom complet carbonic anhydrase III, muscle specific
Masse moléculaire calculée 29 kDa
Poids moléculaire observé 30 kDa
Numéro d’acquisition GenBankBC004897
Symbole du gène CA3
Identification du gène (NCBI) 761
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).

Protocole

Product Specific Protocols
WB protocol for CA3 antibody 15197-1-APDownload protocol
IHC protocol for CA3 antibody 15197-1-APDownload protocol
IF protocol for CA3 antibody 15197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

J Nutr Biochem

Postnatal high-fat diet sex-specifically exacerbates prenatal dexamethasone-induced hypertension: Mass spectrometry-based quantitative proteomic approach.

Authors - Chien-Ning Hsu
mouseIF

Am J Physiol Renal Physiol

Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA+ interstitial cells are fibroblasts

Authors - Dennis R. Clayton

Clin Proteomics

Orthogonal proteomics methods warrant the development of Duchenne muscular dystrophy biomarkers

Authors - Camilla Johansson
mouseWB

PLoS One

Multifactorial Comparative Proteomic Study of Cytochrome P450 2E1 Function in Chronic Alcohol Administration.

Authors - Yuan Wang
  • KD Validated
mouseIHC

PLoS One

Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.

Authors - Claes-Göran Reibring
human,ratWB

FEBS Open Bio

Carbonic anhydrase 3 increases during liver adipogenesis even in pre-obesity, and its inhibitors reduce liver adipose accumulation.

Authors - Hiroyuki Yamamoto

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Elise (Verified Customer) (06-21-2022)

Do not Work for IF with blocking serum 5% and antibody in BSA 1%. Will be tested on IHC and WB and Tested again in IF with 1: 150 dilution

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: kidney tissue