- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-CA3
CA3 Polyclonal Antibody for WB, IF, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF-P, ELISA
Conjugaison
Non conjugué
N° de cat : 15197-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HEK-293, cellules HeLa, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris |
Résultats positifs en IHC | tissu de muscle squelettique de souris, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF-P | tissu de muscle squelettique de souris, tissu rénal humain |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochimie (IHC) | IHC : 1:200-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 1 publications below |
WB | See 3 publications below |
IHC | See 3 publications below |
IF | See 2 publications below |
Informations sur le produit
15197-1-AP cible CA3 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | CA3 Protéine recombinante Ag7344 |
Nom complet | carbonic anhydrase III, muscle specific |
Masse moléculaire calculée | 29 kDa |
Poids moléculaire observé | 30 kDa |
Numéro d’acquisition GenBank | BC004897 |
Symbole du gène | CA3 |
Identification du gène (NCBI) | 761 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).
Protocole
Product Specific Protocols | |
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WB protocol for CA3 antibody 15197-1-AP | Download protocol |
IHC protocol for CA3 antibody 15197-1-AP | Download protocol |
IF protocol for CA3 antibody 15197-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Nutr Biochem Postnatal high-fat diet sex-specifically exacerbates prenatal dexamethasone-induced hypertension: Mass spectrometry-based quantitative proteomic approach. | ||
Am J Physiol Renal Physiol Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA+ interstitial cells are fibroblasts | ||
Clin Proteomics Orthogonal proteomics methods warrant the development of Duchenne muscular dystrophy biomarkers | ||
PLoS One Multifactorial Comparative Proteomic Study of Cytochrome P450 2E1 Function in Chronic Alcohol Administration.
| ||
PLoS One Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation. | ||
FEBS Open Bio Carbonic anhydrase 3 increases during liver adipogenesis even in pre-obesity, and its inhibitors reduce liver adipose accumulation. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Elise (Verified Customer) (06-21-2022) | Do not Work for IF with blocking serum 5% and antibody in BSA 1%. Will be tested on IHC and WB and Tested again in IF with 1: 150 dilution
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