Anticorps Polyclonal de lapin anti-CA13

CA13 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 16696-1-AP

Synonymes

CA XIII, CA13, Carbonate dehydratase XIII, Carbonic anhydrase 13, carbonic anhydrase XIII, CAXIII

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 16696-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16696-1-AP (CA13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 16696-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16696-1-AP (CA13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 16696-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 16696-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CA13 antibody (16696-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 16696-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 16696-1-AP (CA13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu cardiaque humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

16696-1-AP cible CA13 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CA13 Protéine recombinante Ag10086
• Nom complet: carbonic anhydrase XIII
• Masse moléculaire calculée: 262 aa, 29 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC052602
• Symbole du gène: CA13
• Identification du gène (NCBI): 377677
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carbonic anhydrases (CAs) are zinc-containing metalloenzymes that catalyze the reversible hydration of carbon dioxide. The mammalian α-CA gene family has been reported to include at least eleven enzymatically active isoforms with different structural and catalytic properties. Four of the active CA isozymes are cytosolic (CA I, II, III, and VII), four are membrane-associated(CA IV, IX, XII, and XIV), two are mitochondrial (CA VA and VB), and one is a secretory form (CA VI). CA13 is a cytosolic protein that may play a role in embryogenesis and perturbation of its function by genetic modification could potentially lead to developmental abnormalities(PMID:14600151).

Publications

Species	Application	Title
mouse	IF	BMC Med Genomics; Single-cell RNA-sequencing of stria vascularis cells in the adult Slc26a4-/- mouse; Authors - Jin-Young Koh