Anticorps Polyclonal de lapin anti-C4 Alpha Chain/C4b/C4d

• Phare
• Validé par KD/KO

C4 Alpha Chain/C4b/C4d Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 22233-1-AP

Synonymes

Acidic complement C4, C4, C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, Complement C4 A, CPAMD2, RG

---

Galerie de données de validation

• 

  WB analysis using 22233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22233-1-AP (C4 Alpha Chain/C4b/C4d antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 22233-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 22233-1-AP (C4 alpha chain/C4b/C4d antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22233-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22233-1-AP (C4 Alpha Chain/C4b/C4d antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 22233-1-AP

  IP result of anti-C4 Alpha Chain/C4b/C4d (IP:22233-1-AP, 4ug; Detection:22233-1-AP 1:1000) with L02 cells lysate 2000ug.

• 

  IHC staining of human liver using 22233-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22233-1-AP (C4 alpha chain/C4b/C4d antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 22233-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22233-1-AP (C4 alpha chain/C4b/C4d antibody) at dilution of 1:200 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules L02, sang humain, Sanguin humain, tissu hépatique de souris
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:4000-1:40000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

22233-1-AP cible C4 Alpha Chain/C4b/C4d dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C4 Alpha Chain/C4b/C4d Protéine recombinante Ag17583
• Nom complet: complement component 4A (Rodgers blood group)
• Masse moléculaire calculée: 1744 aa, 193 kDa
• Poids moléculaire observé: 80-95 kDa, 200 kDa
• Numéro d’acquisition GenBank: BC063289
• Symbole du gène: C4 Gamma Chain
• Identification du gène (NCBI): 720
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1017-1336aa of human C4-A protein can recognize C4 precursor, C4 alpha chain, C4b, and C4d.

Publications

Species	Application	Title
		Brain; Extracellular vesicle biomarkers for complement dysfunction in schizophrenia; Authors - Ting Xue
mouse	WB,CoIP	Free Radic Biol Med; Complement components regulates ferroptosis in CVB3 viral myocarditis by interatction with TFRC; Authors - Lu Yi ; KD Validated
mouse	IHC	Cell Biochem Funct; In silico analysis and verification of critical genes related to vascular calcification in multiple diseases; Authors - Shicheng Li
human	WB	Immunobiology; Inefficient and abortive classical complement pathway activation by the calcium inositol hexakisphosphate component of the Echinococcus granulosus laminated layer.; Authors - Anabella A Barrios
human	WB	Transplant Direct; Circulating Donor Heart Exosome Profiling Enables Noninvasive Detection of Antibody-mediated Rejection.; Authors - Robert W Hu