Anticorps Polyclonal de lapin anti-MGME1

• Phare
• Validé par KD/KO

MGME1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 23178-1-AP

Synonymes

C20orf72, DDK1, MGME1

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Galerie de données de validation

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  WB analysis of Jurkat using 23178-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23178-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23178-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 23178-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 23178-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23178-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A549, cellules HEK-293, cellules HepG2, cellules Y79, tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

23178-1-AP cible MGME1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MGME1 Protéine recombinante Ag19644
• Nom complet: chromosome 20 open reading frame 72
• Masse moléculaire calculée: 344 aa, 39 kDa
• Poids moléculaire observé: 36-39 kDa
• Numéro d’acquisition GenBank: BC016869
• Symbole du gène: MGME1
• Identification du gène (NCBI): 92667
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MGME1, also named as C20orf72, is a single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. It has preference for 5'-3' exonuclease activity. MGME1 cleaves single-stranded DNA (ssDNA) and several DNA or DNA-RNA chimeric oligonucleotides that showed free nucleic acid ends. It probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair. This antibody detects the 35-40 kDa protein.

Publications

Species	Application	Title
mouse	WB	Immunity; Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling.; Authors - Hongxu Xian ; KD Validated
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu ; KO Validated
mouse	IF,IP	mBio; Heat Shock Protein 60 Is Involved in Viral Replication Complex Formation and Facilitates Foot and Mouth Virus Replication by Stabilizing Viral Nonstructural Proteins 3A and 2C; Authors - Jianli Tang
human	WB	Mol Cell; The proteomic landscape of genotoxic stress-induced micronuclei; Authors - Kate M MacDonald