Anticorps Polyclonal de lapin anti-PPDPF

PPDPF Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 19912-1-AP

Synonymes

C20orf149, EXPDF, PPDPF

---

Galerie de données de validation

• 

  WB analysis using 19912-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19912-1-AP (PPDPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 19912-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19912-1-AP (PPDPF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 19912-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19912-1-AP (PPDPF antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 19912-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19912-1-AP (PPDPF antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 19912-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19912-1-AP (PPDPF antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 19912-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19912-1-AP (PPDPF antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of U2OS using 19912-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PPDPF antibody (19912-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules MCF-7, tissu pancréatique de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

19912-1-AP cible PPDPF dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPDPF Protéine recombinante Ag13740
• Nom complet: chromosome 20 open reading frame 149
• Masse moléculaire calculée: 114 aa, 12 kDa
• Poids moléculaire observé: 10-12 kDa
• Numéro d’acquisition GenBank: BC002531
• Symbole du gène: PPDPF
• Identification du gène (NCBI): 79144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pancreatic progenitor cell differentiation and proliferation factor (PPDPF), also known as C20orf149 and EXPDF, belongs to the PPDPF family. PPDPF is a key regulator for the development of exocrine pancreas. PPDPF is highly expressed in various cancers, including HCC, colorectal cancer, prostate cancer, etc (PMID: 34031390, 34975328, 37027301). PPDPF consists of 114 amino acids and the molecular weight is 12 kDa.

Publications

Species	Application	Title
		Cell Metab; Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.; Authors - Marcel Morgenstern
human,mouse	WB,IHC	Cell Rep; PPDPF suppresses the development of hepatocellular carcinoma through TRIM21-mediated ubiquitination of RIPK1; Authors - Yi-Kang Wang
human	WB	Int J Biol Sci; PPDPF Promotes the Progression and acts as an Antiapoptotic Protein in Non-Small Cell Lung Cancer.; Authors - Mu Yun
human	WB	Front Oncol; The Exocrine Differentiation and Proliferation Factor (EXDPF) Gene Promotes Ovarian Cancer Tumorigenesis by Up-Regulating DNA Replication Pathway.; Authors - Yangjiong Xiao
human	WB,IHC	Medicine (Baltimore); Pancreatic progenitor cell differentiation and proliferation factor predicts poor prognosis in heptaocellular carcinoma.; Authors - Zhengfa Mao
human	WB,IHC,IF	EMBO Rep; Phosphorylation of PPDPF via IL6-JAK2 activates the Wnt/β-catenin pathway in colorectal cancer; Authors - Yuyuan Zi