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Anticorps Polyclonal de lapin anti-NCBP3

NCBP3 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 24384-1-AP

Synonymes

C17orf85, ELG, HSA277841, NCBP3, Protein ELG

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using NCBP3 Polyclonal antibody (24384-1-AP)

    WB analysis using 24384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24384-1-AP (NCBP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NCBP3 Polyclonal antibody (24384-1-AP)

    IF Staining of HeLa using 24384-1-AP

    Immunofluorescent analysis of HeLa cells using 24384-1-AP (NCBP3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Applications testées

Résultats positifs en WBcellules A549, cellules HeLa, cellules Jurkat, cellules PC-3
Résultats positifs en IF/ICCcellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 1 publications below

Informations sur le produit

24384-1-AP cible NCBP3 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène NCBP3 Protéine recombinante Ag20021
Nom complet chromosome 17 open reading frame 85
Masse moléculaire calculée 620 aa, 70 kDa
Poids moléculaire observé 71-80 kDa
Numéro d’acquisition GenBankBC148856
Symbole du gène NCBP3/C17orf85
Identification du gène (NCBI) 55421
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

chromosome 17 open reading frame 85(C17orf85)also named as Protein ELG is 620 amino acids protein, which is localizes in human chromosome 17. The function of protein is still non-known and it consists of some phosphorylation site . It may involves in human embryonic stem cell differentiation.

Protocole

Product Specific Protocols
WB protocol for NCBP3 antibody 24384-1-APDownload protocol
IF protocol for NCBP3 antibody 24384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

RNA Biol

NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells.

Authors - Xiwen Li
View Article

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Hana (Verified Customer) (08-20-2019)

Please make more concentrated Ab.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 5% milk
  • Cell Tissue Type: C2C12 myoblast