Anticorps Monoclonal anti-BrdU

BrdU Monoclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris et plus (1)

Applications

IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

65

CloneNo.

1B10E12

N° de cat : 66241-1-Ig

Synonymes

5-bromo-2-deoxyuridine, BrDu, BrdUrd, Bromodeoxyuridine, BUdR

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  IHC staining of mouse spleen using 66241-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide (with/without BrdU feeding) using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 66241-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (with/without BrdU feeding) slide using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 66241-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (with/without BrdU feeding) slide using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L-929 using 66241-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed L-929 cells using BrdU antibody (66241-1-Ig, Clone: 1B10E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66241-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 66241-1-Ig (BrdU antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66241-1-Ig

  Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using 66241-1-Ig(BrdU antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules L-929

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 12 publications below
• IF: See 40 publications below
• FC: See 1 publications below

Informations sur le produit

66241-1-Ig cible BrdU dans les applications de IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Composé
• Nom complet: BrdU
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has 0mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

Protocol for IHC:
https://www.ptgcn.com/protocol/66241-1-IgIHC.pdf

Protocol for IF:
https://www.ptgcn.com/protocol/66241-1-IgIF.pdf

Publications

Species	Application	Title
mouse	IHC,IF	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
mouse	IF	Sci Transl Med; FoxO transcription factors modulate autophagy and proteoglycan 4 in cartilage homeostasis and osteoarthritis.; Authors - Tokio Matsuzaki
mouse	IF	Cell Res; A novel m6A reader Prrc2a controls oligodendroglial specification and myelination.; Authors - Rong Wu
human	IF	Nat Commun; MRNIP condensates promote DNA double-strand break sensing and end resection.; Authors - Yun-Long Wang
mouse	IF	Nat Commun; Replication stress triggers microsatellite destabilization and hypermutation leading to clonal expansion in vitro.; Authors - Yusuke Matsuno
mouse	IHC	Bone Res; Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx-/- mice.; Authors - Junxin Lin

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