Anticorps Polyclonal de lapin anti-BUB1

• Phare
• Validé par KD/KO

BUB1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 13330-1-AP

Synonymes

Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, EC:2.7.11.1, BUB1L, BUB1A

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Galerie de données de validation

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  WB analysis of MKN-45 using 13330-1-AP

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 13330-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13330-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 13330-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of IMR-90 cells using 13330-1-AP

  IF results of BUB1 anitbody with IMR-90 cells by M.S. Grasiella Angelina Andriani Cristina Montagna Lab at Albert Einstein College of Medicine-Bronx - NY.

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  IF Staining of MDCK using 13330-1-AP

  Immunofluorescent analysis of MDCK cells using 13330-1-AP (BUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa
• Résultats positifs en IF/ICC: cellules IMR-90, cellules MDCK

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

13330-1-AP cible BUB1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BUB1 Protéine recombinante Ag4138
• Nom complet: budding uninhibited by benzimidazoles 1 homolog (yeast)
• Masse moléculaire calculée: 1085 aa, 122 kDa
• Poids moléculaire observé: 45 kDa, 120 kDa
• Numéro d’acquisition GenBank: BC028201
• Symbole du gène: BUB1
• Identification du gène (NCBI): 699
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).

Publications

Species	Application	Title
human		Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan
human	IHC,IF	Cancer Sci; Stathmin guides personalized therapy in oral squamous cell carcinoma.; Authors - Wu-Tong Ju
Carassius auratus	WB	Front Bioeng Biotechnol; A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish.; Authors - Wenting Xu
human	WB	J Exp Clin Cancer Res; Circ_0004087 interaction with SND1 promotes docetaxel resistance in prostate cancer by boosting the mitosis error correction mechanism.; Authors - Liang Chen
human	WB	Front Pharmacol; Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis; Authors - Xie Mengyan
human	WB,IHC	Thorac Cancer; Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma; Authors - Rui Chen ; KD Validated