Anticorps Polyclonal de lapin anti-BTN2A2

BTN2A2 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 16482-1-AP

Synonymes

BT2.2, BTF2, BTN2A2

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 16482-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16482-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 16482-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16482-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16482-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16482-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BTN2A2 antibody (16482-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules MCF-7, cellules NIH/3T3, tissu testiculaire humain
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16482-1-AP cible BTN2A2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BTN2A2 Protéine recombinante Ag9603
• Nom complet: butyrophilin, subfamily 2, member A2
• Masse moléculaire calculée: 523 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC014021
• Symbole du gène: BTN2A2
• Identification du gène (NCBI): 10385
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Butyrophilins constitute a family of transmembrane proteins consisting of butyrophilin (BTN), BTN-like (BTNL), and selection and upkeep of intraepithelial T cell (SKI NT) proteins. It has been reported that BTN2A2 is expressed on B cells, DCs, and macrophages and the expression levels are upregulated upon activation. BTN2A2 is also expressed on thymic epithelial cells (TECs). The BTN2A2 putative receptor is expressed on activated T cells. BTN2A2 protein inhibits T cell proliferation, metabolism and Th1 cytokine production(PMID: 34588505). The calculated MW of BTN2A2 is 59kDa, and the glycosylated form is around 65kDa (PMID:20208008).