Anticorps Monoclonal anti-BST2

BST2 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3C4B5

N° de cat : 66919-1-Ig

Synonymes

Bone marrow stromal antigen 2, BST 2, BST2, CD317, HM1.24 antigen, Tetherin

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Galerie de données de validation

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  WB analysis using 66919-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66919-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66919-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66919-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood leukocyte using 66919-1-Ig

  Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66919-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66919-1-Ig (BST2 antibody) at dilution of 1:500 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human liver using 66919-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66919-1-Ig (BST2 antibody) at dilution of 1:500 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules L02, cellules THP-1, leucocyte de sang périphérique humain
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66919-1-Ig cible BST2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BST2 Protéine recombinante Ag4430
• Nom complet: bone marrow stromal cell antigen 2
• Masse moléculaire calculée: 180 aa, 20 kDa
• Poids moléculaire observé: 18 kDa, 30-36 kDa
• Numéro d’acquisition GenBank: BC033873
• Symbole du gène: BST2
• Identification du gène (NCBI): 684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).

Publications

Species	Application	Title
human		Mol Biol Cell; ER-to-Golgi trafficking of procollagen III via conventional vesicular and tubular carriers.; Authors - Yukihiro Hirata