Anticorps Polyclonal de lapin anti-CD147

CD147 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

33

Avis (1)

N° de cat : 11989-1-AP

Synonymes

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF

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Galerie de données de validation

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  WB analysis of human liver using 11989-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11989-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11989-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11989-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11989-1-AP

  IP result of anti-CD147 (IP:11989-1-AP, 4ug; Detection:11989-1-AP 1:10000) with HEK-293 cells lysate 1200 ug.

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  IHC staining of human lung cancer using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11989-1-AP (CD147 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11989-1-AP (CD147 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11989-1-AP (CD147 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11989-1-AP (CD147 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules Jurkat, tissu cardiaque de souris, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain, tissu de cancer du sein humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Informations sur le produit

11989-1-AP cible CD147 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD147 Protéine recombinante Ag2619
• Nom complet: basigin (Ok blood group)
• Masse moléculaire calculée: 385 aa, 42 kDa
• Poids moléculaire observé: 42-50 kDa
• Numéro d’acquisition GenBank: BC009040
• Symbole du gène: CD147
• Identification du gène (NCBI): 682
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion and an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).

Publications

Species	Application	Title
human	WB	Nat Commun; Genome-wide screens identify SEL1L as an intracellular rheostat controlling collagen turnover; Authors - Michael J Podolsky
human	WB	J Clin Invest; Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders; Authors - Huilun Helen Wang
mouse	CoIP	Cell Mol Immunol; CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ.; Authors - Ruo Chen
mouse	IHC	Acta Pharmacol Sin; 124I-labeled anti-CD147 antibody for noninvasive detection of CD147-positive pan-cancers: construction and preclinical studies; Authors - Xiao-Kun Ma
human	WB	Elife; Erythrocyte invasion-neutralising antibodies prevent Plasmodium falciparum RH5 from binding to basigin-containing membrane protein complexes; Authors - Abhishek Jamwal
human	WB	PLoS Pathog; Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.; Authors - Tao Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mohammed (Verified Customer) (08-19-2020)

Stains the sperm tail very nicely.

• Applications: Immunofluorescence
• Primary Antibody Dilution: (1:200)
• Cell Tissue Type: Testis