Anticorps Polyclonal de lapin anti-BRSK2

• Phare
• Validé par KD/KO

BRSK2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11589-1-AP

Synonymes

BR serine/threonine kinase 2, BRSK2, C11orf7, PEN11B, SAD1, STK29

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Galerie de données de validation

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  WB analysis using 11589-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11589-1-AP (BRSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11589-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11589-1-AP (BRSK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11589-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11589-1-AP (BRSK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11589-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11589-1-AP (BRSK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

11589-1-AP cible BRSK2 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BRSK2 Protéine recombinante Ag2195
• Nom complet: BR serine/threonine kinase 2
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 80-88 kDa
• Numéro d’acquisition GenBank: BC024291
• Symbole du gène: BRSK2
• Identification du gène (NCBI): 9024
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRSK2 is highly conserved from invertebrates to human, plays a key role in polarization of neurons and has been identified with different names: SAD1 is its closest homolog in C. elegans, serine/threonine kinase SADA alfa in rat, brain-selective kinase 2 and SAD-B in mouse and hsSAD1 and SAD1B in humans(PMID:16165222) . In rat and mouse, BRSK2 is only detected in the brain and at low levels in the testis .It has some isoforms produced by alternative splicing with the molecular mass of 68-84 kDa. This protein has a conserved function throughout the evolution.

Publications

Species	Application	Title
rat	WB	J Biol Chem; Specification of neuronal polarity regulated by local translation of CRMP2 and Tau via the mTOR-p70S6K pathway.; Authors - Morita Tsuyoshi T
human,rat	WB	J Neurochem; Phosphorylation of microtubule-associated protein tau by AMPK-related kinases.; Authors - Yoshida Hirotaka H
rat	WB,IP	Endocrinology; SAD-A Promotes Glucose-Stimulated Insulin Secretion Through Phosphorylation and Inhibition of GDIα in Male Islet β Cells.; Authors - Jia Nie ; KD Validated
human,mouse	WB,IF,IP	Cancer Lett; SOD1-high fibroblasts derived exosomal miR-3960 promotes cisplatin resistance in triple-negative breast cancer by suppressing BRSK2-mediated phosphorylation of PIMREG; Authors - Kangdi Li ; KD Validated