Anticorps Polyclonal de lapin anti-BRSK1

BRSK1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 12673-1-AP

Synonymes

BR serine/threonine kinase 1, BRSK1, KIAA1811, SAD1, SAD1 kinase

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Galerie de données de validation

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  WB analysis using 12673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12673-1-AP (BRSK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12673-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12673-1-AP (BRSK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12673-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12673-1-AP (BRSK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12673-1-AP

  IP result of anti-BRSK1 (IP:12673-1-AP, 4ug; Detection:12673-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of human ovary tumor using 12673-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12673-1-AP (BRSK1 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

12673-1-AP cible BRSK1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BRSK1 Protéine recombinante Ag3357
• Nom complet: BR serine/threonine kinase 1
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC016681
• Symbole du gène: BRSK1
• Identification du gène (NCBI): 84446
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRSK1(Brain-specific serine/threonine-protein kinase 1) is also named as KIAA1811, SAD1, SADB and belongs to the SNF1 subfamily. BRSKs are crucial for establishing neuronal polarity, and BRSK1 has also been shown to regulate neurotransmitter release presynaptically. BRSK1 is a serine-threonine kinases specifically expressed in the brain, and activated by LKB1-mediated phosphorylation of a threonine residue at their T-loop. (PMID:22020259). It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
rat	WB	J Biol Chem; Specification of neuronal polarity regulated by local translation of CRMP2 and Tau via the mTOR-p70S6K pathway.; Authors - Morita Tsuyoshi T
goat	WB	Cell Death Discov; Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers; Authors - Zhimin Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ester (Verified Customer) (04-21-2021)

Worked well for Immunofluoresence.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: OVCAR8,PEO4