Anticorps Polyclonal de lapin anti-BRIP1(N-terminal)

• Phare
• Validé par KD/KO

BRIP1(N-terminal) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 24436-1-AP

Synonymes

BACH1, BRIP1, BRIP1(N-terminal), FANCJ, OF

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Galerie de données de validation

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  WB analysis using 24436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24436-1-AP (BRIP1(N-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24436-1-AP

  WB result of BRIP1(N-terminal) antibody (24436-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BRIP1(N-terminal) transfected HeLa cells.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

24436-1-AP cible BRIP1(N-terminal) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BRIP1(N-terminal) Protéine recombinante Ag19907
• Nom complet: BRCA1 interacting protein C-terminal helicase 1
• Masse moléculaire calculée: 1249 aa, 141 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC101472
• Symbole du gène: BRIP1
• Identification du gène (NCBI): 83990
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRIP1 also named as BACH1, FANCJ or ATP dependent RNA helicase BRIP1 is a 1249 amino acid protein, which contains one helicase ATP-binding domain and belongs to the DEAD box heilicase family, DEAH subfamily. BRIP1 contains nuclear localization signal and is ubiquitously expressed with highest levels in testis, an expression pattern similar to that of BRCA1. BRIP1 is required to resolve the secondary structures of guanine-rich DNA that occasionally form during lagging-strand DNA synthesis.

Publications

Species	Application	Title
human	WB	Mol Cell; A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.; Authors - Jiao Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Adrianna (Verified Customer) (10-31-2022)

This antibody is not as good as some of the other Proteintech antibodies (it has more background) and it looks like I am detecting two bands, however I can clearly see the correct band at around 140 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7