Anticorps Polyclonal de lapin anti-BPGM

BPGM Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 17173-1-AP

Synonymes

2,3 bisphosphoglycerate mutase, Bisphosphoglycerate mutase, BPG dependent PGAM, BPGM

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Galerie de données de validation

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  WB analysis of mouse liver using 17173-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 17173-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17173-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 17173-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 17173-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 17173-1-AP

  IP result of anti-BPGM (IP:17173-1-AP, 4ug; Detection:17173-1-AP 1:700) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human liver cancer using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules K-562, cellules LNCaP, cellules MG U-87, plasma humain, tissu cardiaque de souris, tissu hépatique de rat, tissu hépatique de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

17173-1-AP cible BPGM dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BPGM Protéine recombinante Ag10731
• Nom complet: 2,3-bisphosphoglycerate mutase
• Masse moléculaire calculée: 259 aa, 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC017050
• Symbole du gène: BPGM
• Identification du gène (NCBI): 669
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB,IP	Cell Metab; Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis.; Authors - Ping Xu
rat	WB,IF	Brain Res Bull; Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2; Authors - Guoji E
rat	WB	Front Physiol; Alteration in the number, morphology, function, and metabolism of erythrocytes in high-altitude polycythemia; Authors - Song Yu