Anticorps Polyclonal de lapin anti-BMP7

• Phare
• Validé par KD/KO

BMP7 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 12221-1-AP

Synonymes

BMP 7, BMP7, BMP-7, bone morphogenetic protein 7, Eptotermin alfa, OP 1, OP1, Osteogenic protein 1

---

Galerie de données de validation

• 

  WB analysis using 12221-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12221-1-AP (BMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12221-1-AP

  WB result of BMP7 antibody (12221-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMP7 transfected HEK-293 cells.

• 

  IP experiment of HEK-293 using 12221-1-AP

  IP result of anti-BMP7 (IP:12221-1-AP, 4ug; Detection:12221-1-AP 1:800) with HEK-293 cells lysate 2800ug.

• 

  IHC staining of mouse brain using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12221-1-AP (BMP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12221-1-AP (BMP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bladder using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bladder using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 12221-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BMP7 antibody (12221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cérébral de souris, tissu de carcinome à cellules rénales humain, tissu rénal humain, tissu vésical humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 8 publications below

Informations sur le produit

12221-1-AP cible BMP7 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BMP7 Protéine recombinante Ag2902
• Nom complet: bone morphogenetic protein 7
• Masse moléculaire calculée: 431 aa, 49 kDa
• Poids moléculaire observé: 43-49 kDa
• Numéro d’acquisition GenBank: BC008584
• Symbole du gène: BMP7
• Identification du gène (NCBI): 655
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.

BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).

Publications

Species	Application	Title
	WB	Circ Res; Yin Yang 1 Suppresses Dilated Cardiomyopathy and Cardiac Fibrosis Through Regulation of Bmp7 and Ctgf.; Authors - Chia Yee Tan
	WB,IF	Cell Mol Life Sci; Omentin-1 drives cardiomyocyte cell cycle arrest and metabolic maturation by interacting with BMP7; Authors - Huijun Yang
rat	WB	Nanoscale; Concentrated small extracellular vesicles from menstrual blood-derived stromal cells improve intrauterine adhesion, a pre-clinical study in a rat model.; Authors - Siwen Zhang
	WB	Front Mol Neurosci; Tcf4 Controls Neuronal Migration of the Cerebral Cortex through Regulation of Bmp7.; Authors - Tianda Chen
rat	WB,IHC,IF	Front Pharmacol; BMP-7 Upregulates Id2 Through the MAPK Signaling Pathway to Improve Diabetic Tubulointerstitial Fibrosis and the Intervention of Oxymatrine.; Authors - Yawen Xiao
	WB	BMC Complement Med Ther; Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway; Authors - Ying Chen