Anticorps Polyclonal de lapin anti-BMP15

BMP15 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 18982-1-AP

Synonymes

BMP 15, BMP15, BMP-15, bone morphogenetic protein 15, GDF 9B, GDF9B, ODG2, POF4

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Galerie de données de validation

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  WB analysis of HeLa using 18982-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18982-1-AP (BMP15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 18982-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18982-1-AP (BMP15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 18982-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18982-1-AP (BMP15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 18982-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18982-1-AP (BMP15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 18982-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18982-1-AP (BMP15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

18982-1-AP cible BMP15 dans les applications de WB, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: bone morphogenetic protein 15
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: NM_005448
• Symbole du gène: BMP15
• Identification du gène (NCBI): 9210
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BMP15, also named as GDF9B, is a member of the bone morphogenetic protein family which is part of the transforming growth factor beta superfamily. The transforming growth factor beta superfamily includes large families of growth and differentiation factors. BMP15 may be involved follicular development and oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth. Mutation of BMP15 will cause of ovarian dysgenesis type 2 (ODG2) and premature ovarian failure type 4 (POF4).

Publications

Species	Application	Title
human	WB,CoIP	Cell Commun Signal; GDF9His209GlnfsTer6/S428T and GDF9Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement; Authors - Yuwei Duan
human	WB	Mol Med Rep; Abnormal expression levels of BMP15/Smad1 are associated with granulosa cell apoptosis in patients with polycystic ovary syndrome.; Authors - Xiangrong Cui
mouse	IHC	Proc Natl Acad Sci U S A; A rare germline BMP15 missense mutation causes hereditary ovarian immature teratoma in human; Authors - Yakun Liu
mouse	WB	Adv Sci (Weinh); AMPK Suppression Due to Obesity Drives Oocyte mtDNA Heteroplasmy via ATF5-POLG Axis; Authors - Yanting Chen