Anticorps Polyclonal de lapin anti-BMF

• Phare
• Validé par KD/KO

BMF Polyclonal Antibody for IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 18298-1-AP

Synonymes

FLJ00065, Bcl-2-modifying factor

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Galerie de données de validation

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  IP experiment of HEK-293 using 18298-1-AP

  IP result of anti-BMF (IP:18298-1-AP, 4ug; Detection:18298-1-AP 1:1000) with HEK-293 cells lysate 1800 ug.

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Applications testées

• Résultats positifs en IP: cellules HEK-293,

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

18298-1-AP cible BMF dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Bcl2 modifying factor
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC069505
• Symbole du gène: BMF
• Identification du gène (NCBI): 90427
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BMF belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities such as central regulators of apoptotic cell death. BMF contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. BMF is widely expressed in tissues such as pancreas, liver and kidney, and in hematopoietic tissues.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Characterization of an alternative BAK-binding site for BH3 peptides.; Authors - Kaiqin Ye
human	WB	Int J Biol Sci; RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression.; Authors - Feng Xu ; KD Validated
human	WB	PLoS One; Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli.; Authors - Yu Guohua G
mouse	WB	Aging Dis; BMF-AS1/BMF Promotes Diabetic Vascular Calcification and Aging both In Vitro and In Vivo; Authors - Xiao Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chi (Verified Customer) (09-28-2019)

This antibody generally works well to detect BMF in western blot at a relatively high concentration.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse embryonic fibroblasts

BMF Antibody Western Blot, validation (1:500 dilution) in mouse embryonic fibroblasts (Cat no:18298-1-AP)