Anticorps Polyclonal de lapin anti-BLMH

BLMH Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14941-1-AP

Synonymes

BH, bleomycin hydrolase, BLM hydrolase, BLMH, BMH

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Galerie de données de validation

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  WB analysis of human colon using 14941-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14941-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14941-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skin using 14941-1-AP

  IP result of anti-BLMH (IP:14941-1-AP, 4ug; Detection:14941-1-AP 1:1000) with mouse skin tissue lysate 3200ug.

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  IHC staining of human colon cancer using 14941-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14941-1-AP (BLMH antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 14941-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14941-1-AP (BLMH antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14941-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14941-1-AP (BLMH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14941-1-AP

  Immunofluorescent analysis of Hela cells, using BLMH antibody 14941-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu de côlon humain, tissu pancréatique de souris, tissu splénique de souris
• Résultats positifs en IP: tissu cutané de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14941-1-AP cible BLMH dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BLMH Protéine recombinante Ag6888
• Nom complet: bleomycin hydrolase
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC003616
• Symbole du gène: BLMH
• Identification du gène (NCBI): 642
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BLMH(Bleomycin hydrolase) is also named as BH, BMH and belongs to the peptidase C1 family. The gene is expressed in many human tissues with elevated expression levels found in testis, skeletal muscle, and pancreas, and low levels of expression in colon and peripheral blood leukocytes(PMID:8639621). The protein catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. BH-immunoreactivity is essentially located in the cytoplasm and at the outer membrane of the rough endoplasmic reticulum of epithelial cells of the kidney, as well as in that of hepatocytes of the liver(PMID:17158865).

Publications

Species	Application	Title
hamster	IF	BMC Mol Biol; DNA double-strand break repair is impaired in presenescent Syrian hamster fibroblasts.; Authors - Ljudmila Solovjeva
mouse	IHC	Int J Oncol; N6-methyladenosine-induced long non-coding RNA PVT1 regulates the miR-27b-3p/BLM axis to promote prostate cancer progression; Authors - Bin Chen
human	WB	J Dermatol Sci; Increased production of natural moisturizing factors and bleomycin hydrolase activity in elderly human skin; Authors - Munehiro Tsurumachi
human	WB	Cancer Gene Ther; METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1; Authors - Rui Yang