Anticorps Polyclonal de lapin anti-BHLHE41

BHLHE41 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 12688-1-AP

Synonymes

BHLHB3, BHLHE41, DEC2, hDEC2, SHARP 1, SHARP1

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Galerie de données de validation

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  WB analysis using 12688-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12688-1-AP (BHLHE41 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 12688-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using BHLHE41 antibody (12688-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris,
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

12688-1-AP cible BHLHE41 dans les applications de WB, IHC, IF/ICC, IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BHLHE41 Protéine recombinante Ag3381
• Nom complet: basic helix-loop-helix family, member e41
• Masse moléculaire calculée: 482 aa, 51 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC025968
• Symbole du gène: BHLHE41
• Identification du gène (NCBI): 79365
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BHLHE41 (basic helix-loop-helix family member e41), also called SHARP1 and DEC2, is a member of the BHLH family proteins that are involved in differentiation, circadian rhythms and the response to hypoxia. In esophageal, osteosarcomas, prostate, breast, and endometrial cancer cells, BHLHE41 functions as an anti-apoptotic factor and promotes cell proliferation and invasiveness. It suppresses cell proliferation and metastasis, and also facilitates pancreatic as well as gastric cancer cell apoptosis. BHLHE41 has an important role in the proliferation of glioblastoma and couldserve as a novel candidate for targeted therapy of glioblastoma (PMID: 31616182).

Publications

Species	Application	Title
human	chIP	Nat Commun; The basic helix-loop-helix transcription factor SHARP1 is an oncogenic driver in MLL-AF6 acute myelogenous leukemia.; Authors - Akihiko Numata
rat	WB	FASEB J; Persistent myopathy despite release of partial obstruction: in vivo reversal of dysfunction and transcriptional responses using rapamycin.; Authors - Annette Schröder
human	WB	Biochem Biophys Res Commun; Loss of circadian protein TIMELESS accelerates the progression of cellular senescence.; Authors - Xiaomeng Shen
human	WB, IHC	J Exp Clin Cancer Res; DEC2 expression is positively correlated with HIF-1 activation and the invasiveness of human osteosarcomas.; Authors - Tu Hu
human	WB,IHC	J Exp Clin Cancer Res; Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma.; Authors - Xiao Yang
human	WB,IF	Cell Death Dis; Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells.; Authors - Meng Zhang