Anticorps Polyclonal de lapin anti-BCOR

BCOR Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 12107-1-AP

Synonymes

ANOP2, BCOR, KIAA1575, MAA2, MCOPS2

---

Galerie de données de validation

• 

  WB analysis using 12107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12107-1-AP (BCOR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12107-1-AP (BCOR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12107-1-AP

  IP result of anti-BCOR (IP:12107-1-AP, 4ug; Detection:12107-1-AP 1:5000) with HEK-293 cells lysate 920 ug.

• 

  IHC staining of human breast cancer using 12107-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12107-1-AP (BCOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12107-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12107-1-AP (BCOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

12107-1-AP cible BCOR dans les applications de WB, IP, IHC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCOR Protéine recombinante Ag2752
• Nom complet: BCL6 co-repressor
• Masse moléculaire calculée: 1703 aa, 186 kDa
• Poids moléculaire observé: 186 kDa
• Numéro d’acquisition GenBank: BC009675
• Symbole du gène: BCOR
• Identification du gène (NCBI): 54880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCOR, a corepressor of BCL6, binds to transcriptional factors and specifically inhibits gene expression. BCOR plays a key role in the early embryonic development, mesenchymal stem cell function and hematopiesis. BCOR can associate with HDACs, the polycomb group protein, polycomb group Ring finger protein1/nervous system polycomb1 (PCGF1/NSPC1), histone demethylase, F-box- and leucine-rich repeat protein10(FBXL10). Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem

Publications

Species	Application	Title
	WB, IHC	Nat Genet; Consistent in-frame internal tandem duplications of BCOR characterize clear cell sarcoma of the kidney.; Authors - Hitomi Ueno-Yokohata
human	WB	Nucleic Acids Res; Bimodal regulation of the PRC2 complex by USP7 underlies tumorigenesis.; Authors - Dongxue Su
mouse	WB	Int J Mol Sci; BCOR Internal Tandem Duplication Expression in Neural Stem Cells Promotes Growth, Invasion, and Expression of PRC2 Targets.; Authors - Satoshi Nakata
mouse	WB	Sci Rep; Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells.; Authors - Yun Yan
		Cell Prolif; Secreted frizzled-related protein 2 promotes the osteo/odontogenic differentiation and paracrine potentials of stem cells from apical papilla under inflammation and hypoxia conditions.; Authors - Haoqing Yang
human	IHC	J Clin Pathol; Novel exon-exon breakpoint in CIC-DUX4 fusion sarcoma identified by anchored multiplex PCR (Archer FusionPlex Sarcoma Panel).; Authors - Benjamin Nathanael Loke

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Sid (Verified Customer) (05-13-2019)

Works well for mouse hematopoetic cells, some strong non specific bands ~ 150 kD and ~100 kD, however can be reasonably resolved on 4-15% gel.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse hematopoetic