Anticorps Polyclonal de lapin anti-BCKDHB

BCKDHB Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 13685-1-AP

Synonymes

BCKDHB, E1B

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Galerie de données de validation

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  WB analysis using 13685-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13685-1-AP (BCKDHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 13685-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13685-1-AP (BCKDHB Antibody) at dilution of 1:350 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 13685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13685-1-AP (BCKDHB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13685-1-AP (BCKDHB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 13685-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13685-1-AP (BCKDHB antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 13685-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13685-1-AP (BCKDHB antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HEK-293 transfectées, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

13685-1-AP cible BCKDHB dans les applications de WB, RIP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCKDHB Protéine recombinante Ag4552
• Nom complet: branched chain keto acid dehydrogenase E1, beta polypeptide
• Masse moléculaire calculée: 392 aa, 43 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC040139
• Symbole du gène: BCKDHB
• Identification du gène (NCBI): 594
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Branched-chain alpha-keto acid dehydrogenase E1 component beta chain (BCKDHB; BCKDE1B; BCKDH E1-beta) is a subunit of the BCKDH complex, which is a mitochondrial enzyme in the degradation pathway for branched-chain amino acids (BCAA). Together with BCKDHA, BCKDHB forms the E1 subunit of this complex, whereas DBT and DLD are the E2 and E3 subunits, respectively. A deficiency of the BCKDH complex in humans causes maple syrup urine disease (MSUD), a severe neurometabolic disorder diagnosed by the detection alloisoleucine in plasma (MIM 248600). (PMID: 30709776)

Publications

Species	Application	Title
sheep	WB	Anim Sci J; MiR-330-5p negatively regulates ovine preadipocyte differentiation by targeting branched-chain aminotransferase 2.; Authors - Tao Shi
mouse	WB	Cell Rep; SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis.; Authors - Elma Zaganjor
human	WB	Cell Rep; Branched-chain amino acid catabolism breaks glutamine addiction to sustain hepatocellular carcinoma progression; Authors - Dongdong Yang
human	IHC	Nat Commun; AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis; Authors - Yanan Wang
mouse	WB	J Thromb Haemost; Branched-chain amino acids promote thrombocytopoiesis by activating mTOR signaling; Authors - Haojie Jiang
mouse	WB,RIP	Nucleic Acids Res; Mitolnc controls cardiac BCAA metabolism and heart hypertrophy by allosteric activation of BCKDH; Authors - Maria Weiss