• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-BCCIP

BCCIP Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 16043-1-AP

Synonymes

BCCIP, Protein TOK 1, TOK 1, TOK1



Applications testées

Résultats positifs en WBcellules HeLa, cellules A549
Résultats positifs en IPcellules HeLa
Résultats positifs en IF/ICCcellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

16043-1-AP cible BCCIP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène BCCIP Protéine recombinante Ag8934
Nom complet BRCA2 and CDKN1A interacting protein
Masse moléculaire calculée 314 aa, 36 kDa
Poids moléculaire observé 50-58 kDa
Numéro d’acquisition GenBankBC009771
Symbole du gène BCCIP
Identification du gène (NCBI) 56647
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCCIP (or TOK1) is isolated as a BRCA2- and CDKN1A (p21)-interacting protein and is implicated in homologous recombination (HR) pathway and inhibition of DNA replication stress. BCCIP is an evolutionarily conserved nuclear protein with multiple interacting domains. BCCIP deficiency in mice impaired embryonic and postnatal neural development, causing severe ataxia, cerebral and cerebellar defects, and microcephaly, which are associated with spontaneous DNA damage and subsequent cell death in the proliferative cell populations of the neural system during embryogenesis. BCCIP is essential for maintaining the transactivation activity of wild type p53 suggesting a potential role of BCCIP in cancer etiology.

Protocole

Product Specific Protocols
WB protocol for BCCIP antibody 16043-1-APDownload protocol
IF protocol for BCCIP antibody 16043-1-APDownload protocol
IP protocol for BCCIP antibody 16043-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

The Males Absent on the First (MOF) Mediated Acetylation Alters the Protein Stability and Transcriptional Activity of YY1 in HCT116 Cells

Authors - Tingting Wu
humanWB

Int J Mol Sci

Feedback Modulation between Human INO80 Chromatin Remodeling Complex and miR-372 in HCT116 Cells

Authors - Junaid Ali Shah
humanWB,IF

FEBS J

BCCIP binds to and activates its promoter in a YY1-dependent fashion in HCT116 cells.

Authors - Yi Sui
  • KD Validated
humanWB,IF

Protein Cell

Human INO80/YY1 chromatin remodeling complex transcriptionally regulates the BRCA2- and CDKN1A-interacting protein (BCCIP) in cells.

Authors - Jiaming Su
humanWB

Biochem Biophys Res Commun

BCCIPβ facilitates p53 ubiquitination via binding with E6 protein in high-risk HPV positive head and neck squamous cell carcinoma.

Authors - Fang Wang
humanWB,IHC

Int J Oncol

Differential BCCIP gene expression in primary human ovarian cancer, renal cell carcinoma and colorectal cancer tissues.

Authors - Liu Xiaoxia X