Anticorps Polyclonal de lapin anti-BCAS3

• Phare
• Validé par KD/KO

BCAS3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 10402-1-AP

Synonymes

BCAS3, GAOB1, MAAB, Protein Maab1, Rudhira

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Galerie de données de validation

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  WB analysis using 10402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10402-1-AP (BCAS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10402-1-AP

  WB result of BCAS3 antibody (10402-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAS3 transfected A431 cells.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HeLa, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

10402-1-AP cible BCAS3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCAS3 Protéine recombinante Ag0646
• Nom complet: breast carcinoma amplified sequence 3
• Masse moléculaire calculée: 99 kDa
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC001250
• Symbole du gène: BCAS3
• Identification du gène (NCBI): 54828
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rudhira/BCAS3 (Breast Cancer Amplified Sequence 3) is a conserved protein expressed in the embryonic vasculature and malignant tumors. Rudhira is a predicted WD40 domain protein. During mouse development Rudhira is expressed in erythropoietic and angiogenic precursors but not in their differentiated progeny. This transient expression of Rudhira is also seen in normal adult angiogenesis. BCAS3, the human ortholog of Rudhira is 98% identical and the gene maps to a breakpoint of hematological neoplasms on chromosome 17q23.1. BCAS3 is also expressed in vascular development in embryoid bodies in vitro and was originally identified as a gene over-expressed in breast cancers. It is reported to be a target of metastasis associated protein-1 (MTA-1). Further BCAS3 expression is also upregulated in grade III and IV glioblastomas where it is misexpressed in the tumor cells.