Anticorps Polyclonal de lapin anti-BCAS2

• Phare
• Validé par KD/KO

BCAS2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10414-1-AP

Synonymes

BCAS2, DAM1, Pre mRNA splicing factor SPF27

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Galerie de données de validation

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  WB analysis using 10414-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10414-1-AP

  WB result of BCAS2 antibody (10414-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAS2 transfected HeLa cells.

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  WB analysis of SH-SY5Y using 10414-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10414-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10414-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of hESC using 10414-1-AP

  hESC cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10414-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10414-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10414-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 10414-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10414-1-AP

  IP result of anti-BCAS2 (IP:10414-1-AP, 3ug; Detection:10414-1-AP 1:1000) with mouse brain tissue lysate 6500ug.

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  IHC staining of human prostate cancer using 10414-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10414-1-AP (BCAS2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10414-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10414-1-AP (BCAS2 antibody) at dilution of 1:100 (under 25x lens).

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  IHC staining of human breast cancer using 10414-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10414-1-AP (BCAS2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10414-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10414-1-AP (BCAS2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 10414-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10414-1-AP (BCAS2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10414-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10414-1-AP (BCAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 10414-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10414-1-AP (BCAS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules COS-7, cellules K-562, cellules PC-12, cellules SCEh, cellules SH-SY5Y, tissu cérébral de souris, tissu cérébral humain, tissu hépatique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

10414-1-AP cible BCAS2 dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCAS2 Protéine recombinante Ag0669
• Nom complet: breast carcinoma amplified sequence 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26-32 kDa
• Numéro d’acquisition GenBank: BC005285
• Symbole du gène: BCAS2
• Identification du gène (NCBI): 10286
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCAS2 associates with the spliceosome and implicates in mRNA splicing

Publications

Species	Application	Title
mouse	WB	Nat Commun; BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis.; Authors - Wenbo Liu
	WB	Int J Biochem Cell Biol; BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination.; Authors - Shengjie Liao
mouse	WB,IF	Stem Cell Res Ther; Breast carcinoma-amplified sequence 2 regulates adult neurogenesis via β-catenin.; Authors - Hsin-Hsiung Chen ; KD Validated
human	IHC, WB	Br J Cancer; BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability.; Authors - P-C Kuo ; KD Validated
mouse	WB,IF	FASEB J; BCAS2 is involved in alternative splicing and mouse oocyte development.; Authors - Jiaqi Zhang ; KO Validated
mouse	WB,RIP	Endocrinology; BCAS2 participates in insulin synthesis and secretion via mRNA alternative splicing in mice; Authors - Xuexue Chen