Anticorps Polyclonal de lapin anti-BBS10

• Phare
• Validé par KD/KO

BBS10 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12421-2-AP

Synonymes

Bardet Biedl syndrome 10, BBS10, C12orf58

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Galerie de données de validation

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  WB analysis of HepG2 using 12421-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12421-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12421-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 cells using 12421-2-AP

  IF result (nuclear and the base of cilia stain) of anti-BBS10 (12421-2-AP; 1:50) with hTERT-RPE1 cell (PFA fixed) by Dr. Moshe Kim.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules MCF-7
• Résultats positifs en IF/ICC: cellules hTERT-RPE1

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

12421-2-AP cible BBS10 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BBS10 Protéine recombinante Ag3073
• Nom complet: Bardet-Biedl syndrome 10
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: BC013795
• Symbole du gène: BBS10
• Identification du gène (NCBI): 79738
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID:20080638,19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).

Publications

Species	Application	Title
human	WB,IF	Proc Natl Acad Sci U S A; Transient ciliogenesis involving Bardet-Biedl syndrome proteins is a fundamental characteristic of adipogenic differentiation.; Authors - Marion Vincent V ; KD Validated
human,mouse	WB,IF	Proc Natl Acad Sci U S A; BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.; Authors - Seo Seongjin S
mouse	WB	Cilia; Comparing the Bbs10 complete knockout phenotype with a specific renal epithelial knockout one highlights the link between renal defects and systemic inactivation in mice.; Authors - Noëlle Cognard ; KO Validated