Anticorps Polyclonal de lapin anti-DDX39B

• Phare
• Validé par KD/KO

DDX39B Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 14798-1-AP

Synonymes

ATP dependent RNA helicase p47, BAT1, D6S81E, DDX39, DDX39B, DEAD box protein UAP56, HLA B associated transcript 1, Spliceosome RNA helicase BAT1, UAP56

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Galerie de données de validation

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  WB analysis of Jurkat using 14798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 14798-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 14798-1-AP

  IP result of anti-DDX39B (IP:14798-1-AP, 4ug; Detection:14798-1-AP 1:500) with Jurkat cells lysate 1200ug.

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  IHC staining of human breast cancer using 14798-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14798-1-AP (UAP56 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14798-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14798-1-AP (UAP56 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu splénique de rat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

14798-1-AP cible DDX39B dans les applications de WB, IP, IF, IHC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX39B Protéine recombinante Ag6512
• Nom complet: HLA-B associated transcript 1
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC000361
• Symbole du gène: BAT1
• Identification du gène (NCBI): 7919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX39B, is a 428 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain and belongs to the DEAD box helicase family. DECD subfamily. DDX39B localizes in the nucleus and cytoplasm. BAT1 is involved in nuclear export of spliced and unspliced mRNA. BAT1 as ATP dependent RNA helicase, may be a translation initiation factor and acts as a negative regulator of inflammatory cytokines. This antibody may cross-react with DDX39A.

Publications

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
human	IF,ChIP	Genes Dev; UAP56/DDX39B is a major cotranscriptional RNA-DNA helicase that unwinds harmful R loops genome-wide.; Authors - Carmen Pérez-Calero
human,mouse	WB,IHC	Cell Death Discov; DDX39B contributes to the proliferation of colorectal cancer through direct binding to CDK6/CCND1.; Authors - Haonan Zhang ; KD Validated
human	WB	iScience; THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate; Authors - Mareike Polenkowski
pig	WB	ACS Infect Dis; Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2.; Authors - Lin Yang
human	WB,IF,IP	Sci Rep; ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export.; Authors - Ashley Boehringer