Anticorps Polyclonal de lapin anti-BAG1S/1M/1L

• Phare
• Validé par KD/KO

BAG1S/1M/1L Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 16150-1-AP

Synonymes

BAG1, BAG1S/1M/1L

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Galerie de données de validation

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  WB analysis of mouse brain using 16150-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16150-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16150-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16150-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules Jurkat, tissu hépatique de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

16150-1-AP cible BAG1S/1M/1L dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: BCL2-associated athanogene
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 46-50 kDa, 41-46 kDa, 25-33 kDa
• Numéro d’acquisition GenBank: NM_004323
• Symbole du gène: BAG1
• Identification du gène (NCBI): 573
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAG1 has been identified that modulates gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M, and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody recognizes three isoforms of BAG1, includes GAG1L, BAG1M, and BAG1S.

Publications

Species	Application	Title
human	WB,IHC	Tumour Biol; Silencing Bag-1 gene via magnetic gold nanoparticle-delivered siRNA plasmid for colorectal cancer therapy in vivo and in vitro.; Authors - Wenbai Huang ; KD Validated