Anticorps Polyclonal de lapin anti-BAG1

• Phare
• Validé par KD/KO

BAG1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 19064-1-AP

Synonymes

BAG 1, BAG1, BAG1L speicfic, BAG1M, BAG1S, HAP, RAP46

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Galerie de données de validation

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  WB analysis of HeLa using 19064-1-AP

  WB result of BAG1 antibody (19064-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG1 transfected HeLa cells.

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  WB analysis of HeLa using 19064-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of UV treated Hela using 19064-1-AP

  UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 19064-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 19064-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19064-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19064-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19064-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19064-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 19064-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19064-1-AP

  IP result of anti-BAG1 (IP:19064-1-AP, 3ug; Detection:19064-1-AP 1:500) with HeLa cells lysate 3440ug.

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  IHC staining of human testis using 19064-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 19064-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 19064-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 19064-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 19064-1-AP

  Immunofluorescent analysis of HeLa cells, using BAG1 antibody 19064-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of MCF-7 using 19064-1-AP

  Immunofluorescent analysis of MCF-7 cells, using BAG1 antibody 19064-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HL-60, cellules HT-1080, cellules Jurkat, cellules MCF-7, RAW264.7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

19064-1-AP cible BAG1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BAG1 Protéine recombinante Ag13555
• Nom complet: BCL2-associated athanogene
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 50-55 kDa, 30-33 kDa
• Numéro d’acquisition GenBank: BC014774
• Symbole du gène: BAG1
• Identification du gène (NCBI): 573
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody can recognize all the isoforms of BAG1.

Publications

Species	Application	Title
		Oncol Rep; Magnetic gold nanoparticle-mediated small interference RNA silencing Bag-1 gene for colon cancer therapy.; Authors - Wenbai Huang
human	IHC	Ann Rheum Dis; Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration; Authors - Yue Fan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This BAG1 antibody works very well in detecting endogenous BAG1 protein by western blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart