Anticorps Polyclonal de lapin anti-BACE1

• Phare
• Validé par KD/KO

BACE1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

33

Avis (1)

N° de cat : 12807-1-AP

Synonymes

Beta-site amyloid precursor protein cleaving enzyme 1, Beta-secretase 1, Beta secretase 1, Aspartyl protease 2, ASP21

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Galerie de données de validation

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  WB analysis of mouse brain using 12807-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12807-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12807-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12807-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of zebrafish using 12807-1-AP

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12807-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12807-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12807-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12807-1-AP

  IP result of anti-BACE1 (IP:12807-1-AP, 3ug; Detection:12807-1-AP 1:500) with mouse brain tissue lysate 5000ug.

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  IP experiment using 12807-1-AP

  IP result of BACE1 antibody (12807-1-AP for IP and Detection) with mouse brain tissue lysate.

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  IHC staining of mouse brain using 12807-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12807-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12807-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 12807-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 12807-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12807-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 12807-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BACE1 antibody (12807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules PC-12, tissu cérébral de rat, tissu cérébral humain, tissu de poisson-zèbre, tissu pancréatique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 31 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

12807-1-AP cible BACE1 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

• Réactivité: Humain, poisson-zèbre, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BACE1 Protéine recombinante Ag3559
• Nom complet: beta-site APP-cleaving enzyme 1
• Masse moléculaire calculée: 501 aa, 56 kDa
• Poids moléculaire observé: 70-75 kDa, 42-56 kDa
• Numéro d’acquisition GenBank: BC036084
• Symbole du gène: BACE1
• Identification du gène (NCBI): 23621
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).

Publications

Species	Application	Title
human	WB	Small; Brain-Penetration and Neuron-Targeting DNA Nanoflowers Co-Delivering miR-124 and Rutin for Synergistic Therapy of Alzheimer's Disease.; Authors - Qin Ouyang
mouse	WB	Redox Biol; Hyperoside alleviates toxicity of β-amyloid via endoplasmic reticulum-mitochondrial calcium signal transduction cascade in APP/PS1 double transgenic Alzheimer's disease mice; Authors - Lin Lin Song
mouse	WB	Redox Biol; A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease; Authors - Yi Sun
mouse	WB	Proc Natl Acad Sci U S A; Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production.; Authors - Gunjan Joshi
human,mouse	WB	Cell Rep; CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease.; Authors - Yang-Ping Shentu
mouse	WB	Stem Cell Reports; BACE1 Deficiency Causes Abnormal Neuronal Clustering in the Dentate Gyrus.; Authors - Hailong Hou ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaoyu (Verified Customer) (02-07-2024)

Good signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: brain

BACE1 Antibody Western Blot validation (1:1000 dilution) in brain (Cat no:12807-1-AP)